In addition, some nonwoven fabrics can be recycled after use, given the proper treatment and facilities. We are the supplier and exporter of air purifier systems, air sampler systems, colony counters, electric sterilizer. Microsofts Activision Blizzard deal is key to the companys mobile gaming efforts. Wojciechowski K, Chang CH, Hocking DC. Blood accounts for 7% of the human body weight, with an average density around 1060 kg/m 3, very close to pure water's density of 1000 kg/m 3. Plasmids and EBNA mRNA were electroporated into fibroblast cells on Amaxa apparatus according to company instructions. A pipette is a laboratory tool used to transport a measured volume of liquid. [2] Synthetic difficulty also is sequence dependent; typically aggregation-prone sequences such as amyloids[9] are difficult to make. A hormone (from the Greek participle , "setting in motion") is a class of signaling molecules in multicellular organisms that are sent to distant organs by complex biological processes to regulate physiology and behavior. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Ludwig TE, et al. A molecule is an electrically neutral group of two or more atoms held together by chemical bonds. [12] A specially denatured alcohol is a combination of ethanol and another chemical substance, e.g., ethyl acetate in SDA 29, 35, and 35A, added to render the mixture unsuitable for drinking. A chemical platform for improved induction of human iPSCs. This was especially common during various anti-alcohol campaigns initiated by the Soviet government. It is a bright, slightly orange-yellow, dense, soft, malleable, and ductile metal in a pure form. Coffee is a drink prepared from roasted coffee beans.Darkly colored, bitter, and slightly acidic, coffee has a stimulating effect on humans, primarily due to its caffeine content. (*p < 0.05, n = 3, relative to survival in TeSR) (b) The plots show proliferation of cells from (a) 96 hours after plating and culture in the same media with daily media change. Higher Certified Media to Support Establishment of Clinical Applications PROTOCOLS Recommended isolation, 2D/3D culturing, and other protocols Swiss Quality Precision Media CHEMICALLY DEFINED, ANIMAL COMPONENT-FREE. Chung TL, et al. Accessibility The term is used in the textile manufacturing industry to denote fabrics, such as felt, which are neither woven nor knitted. Insoluble material was pelleted by centrifugation at 10,000 g. The pellet was washed once with PBS + 0.5 M NaCl and then solubilized in urea buffer (8 M urea, 20 mM Tris pH 7.6, 150 mM NaCl and 3 mM DTT). Briefly, the E coli pellet was resuspended in PBS and lysed with FastBreak cell lysis reagent (Promega). (c) The plots show cell survival after 24 hours on VTN-NC (right) and matrigel (left) in E8(TGF) media after passaging with EDTA (red) and TrypLE (blue) (*p < 0.05, n = 3). It is also available in Protecting group strategies are usually necessary to prevent undesirable side Pair wise dropout experiments revealed that human ES cells survived without BSA when -mercaptoethanol (BME) was also removed from the medium (Fig. National Library of Medicine (*p < 0.05, n = 3). Cocktails of defined chemical compounds: sufficient to induce totipotency in embryonic stem cells, Chemical journey of somatic cells to pluripotency. Thank you for visiting nature.com. Wet laid mat or "glass tissue" use wet-chopped, heavy denier fibers in the 6 to 20 micrometre diameter range. As an example, the formulation for completely denatured alcohol, according to 2005 British regulations was as follows:[10]. Because vitronectin is relatively easy to over-express and purify14, we tested several vitronectin variants and identified two (VTN-NC and VTN-N) that supported human ES cell attachment and survival better than wild type in E8 medium (Fig. At the end of the synthesis, the crude peptide is cleaved from the solid support while simultaneously removing all protecting groups using a reagent such as trifluoroacetic acid. It is also available in A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Other rocks can be defined by relative abundances of key (essential) minerals; a granite is defined by proportions of quartz, alkali feldspar, and They are flat or tufted porous sheets that are made directly from separate fibres, molten plastic or plastic film. A patterned mask is then applied to the Ancient Egypt was a civilization in ancient Northeast Africa, situated in the Egyptian Nile Valley in Egypt.Ancient Egyptian civilization followed prehistoric Egypt and coalesced around 3100 BC (according to conventional Egyptian chronology) with the political unification of Upper and Lower Egypt under Menes (often identified with Narmer). We have used E8 medium to derive iPS cell lines with both episomal and lentiviral methods from five banked fibroblast cell lines from commercial sources, obtained from individuals ranging in age from neonatal to 38 years of age (Supplementary Table 2). Furthermore, these media are useful in in vitro cell cultures of human and animal cells. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in and chemicals and biochemicals required for cell culture. Evidence that type 1 plasminogen activator inhibitor binds to the somatomedin B domain of vitronectin. Our custom writing service is a reliable solution on your academic journey that will always help you if your deadline is too tight. Prior to the addition of cells, 500 l of medium was loaded into each well. 5d). A specially denatured alcohol (SDA) is one of many types of denatured alcohol specified under the United States Title 27 of the Code of Federal Regulations Section 21.151. HiMedia offers a broad range of classical media, balanced salt solutions, reagents, sera, consumables and plasticware and chemicals and biochemicals required for cell culture. All constructs were verified by sequencing. The beans are roasted and then ground into fine particles that With the exception of the banked neonatal fibroblasts that reprogrammed at a high efficiency, the efficiencies obtained directly from biopsy samples grown in E8-based medium were consistently higher than those of established cell lines obtained from commercial sources, suggesting that fibroblast passage history is important. understand the criticality of your experimental results; [citation needed], The strategy for the solid-phase synthesis of cyclic peptides is not limited to attachment through Asp, Glu or Lys side chains. However, the variation in sources of these media components is substantial, making extensive quality control necessary for all new batches. A t-test was performed to calculate p-values for the difference between the means of the experimental conditions and control. Blood accounts for 7% of the human body weight, with an average density around 1060 kg/m 3, very close to pure water's density of 1000 kg/m 3. In organic chemistry, peptide synthesis is the production of peptides, compounds where multiple amino acids are linked via amide bonds, also known as peptide bonds.Peptides are chemically synthesized by the condensation reaction of the carboxyl group of one amino acid to the amino group of another. Using the E8-based medium, defined conditions can be used for all stages of iPS cell derivation and culture. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. When confluency was reached ~20%, hydrocortisone was removed. The history of ancient Egypt occurred as a Most of these materials are too expensive for large-scale usage. 4a). Because of those results, -mercaptoethanol was included in mouse ES cell media25 and subsequently in early human ES cell media1. (d, e) The plots show cell survival after 24 hours (d) or cloning efficiency (e) on VTN-NC (red) and matrigel (blue) surfaces in the presence of the indicated small chemical inhibitors (*p < 0.05, n = 3). [5], Spunbond nonwovens are made in one continuous process. Carding operations typically use ~1.5" (3.8cm) long fibers. At each time point, cells were again dissociated with 0.4 ml TrypLE, neutralized with equal volumes of 10% FBS in DMEM, harvested with pipettes, and counted by flow cytometry. Some rocks, such as limestone or quartzite, are composed primarily of one mineral calcite or aragonite in the case of limestone, and quartz in the latter case. Individual iPS cell clones were confirmed to be integration-free by PCR and, after about twenty passages, cells had normal karyotypes, expressed pluripotency markers (Fig. RNA-Seq gene expression estimation with read mapping uncertainty. Cells were then picked into individual wells with E8 (TGF or NODAL). It is the computed response of the system or organism to various stimuli or Yu J, et al. It is therefore a weak base , and tends to remain as the fluoride ion rather than generating a substantial amount of hydrogen fluoride. Each protein has its own unique amino acid sequence that is specified by the nucleotide sequence of the gene encoding this protein. Furthermore, these media are useful in in vitro cell cultures of human and animal cells. The process begins by coating a substrate with a light-sensitive organic material. 5c). Molecular Biology's classical period began in 1953, with James Watson and Francis Crick's discovery of the double helical structure of DNA. It plays a role in social bonding, reproduction, childbirth, and the period after childbirth. Open Access [2] The crude peptide can be precipitated from a non-polar solvent like diethyl ether in order to remove organic soluble byproducts. These bridges help to stabilize proteins, especially those secreted from cells. In combination with other materials they provide a spectrum of products with diverse properties, and are used alone or as components of apparel, home furnishings, health care, engineering, industrial and consumer goods. Res. Fiberglass is wetlaid into mats for use in roofing and shingles. Chemistry is the scientific study of the properties and behavior of matter. A patterned mask is then applied to the 1A). These reagents attack the O-acylisourea intermediate to form an active ester, which subsequently reacts with the peptide to form the desired peptide bond. Because reprogramming occurs over time, it is often difficult to cleanly distinguish between these two effects, and indeed, they do not need to be mutually exclusive. A more expensive route uses binder fibers or powders that soften and melt to hold other non-melting fibers together, one type of cotton staple nonwoven is treated with sodium hydroxide to shrink bond the mat, the caustic causes the cellulose-based fibers to curl and shrink around one another as the bonding technique, one unusual polyamide(Cerex) is self-bonded with gas-phase acid. Fragment condensation is also undesirable since the coupled fragment must be in gross excess, which may be a limitation depending on the length of the fragment. The crude peptide can be purified using reversed-phase HPLC. It is the most popular hot drink in the world. The resin can be washed between each steps. (2022)Cite this article. Fragment condensation is better than stepwise elongation for synthesizing sophisticated long peptides, but its use must be restricted in order to protect against racemization. Individual iPS cell colonies were selected and subsequently passaged and maintained in complete E8 medium for long-term expansion. A binary star is a system of two stars that are gravitationally bound to and in orbit around each other. In spite of the much greater simplicity of E8 medium, it supports the long-term undifferentiated proliferation of both human ES and iPS cells comparably to TeSR (Fig. As seen in the chart to the right, rare-earth elements are found on earth at similar concentrations to many common transition metals. Cells were then picked into individual wells with E8 (TGF or NODAL). The results of these tests are used for selection of the most appropriate antimicrobial agent(s) for treatment against the infectious organisms. Culture conditions for human embryonic stem cells. Chemically Defined Components Offering Accurate and Highly Reproducible 2D/3D Models. The solid support consists of small, polymeric resin beads functionalized with reactive groups (such as amine or hydroxyl groups) that link to the nascent peptide chain. Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. Purification of vitronectin and its variants was done using a protocol modified from a previous report33. Scavengers such as water and triisopropylsilane (TIPS) are added during the final cleavage in order to prevent side reactions with reactive cationic species released as a result of side chain deprotection. 1d). Here, we demonstrate induction and long-term maintenance of TotiSCs from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. After expansion, the fibroblasts were subjected to the episomal reprogramming procedure described above (Fig. is a founder, stockowner, consultant and board member of Cellular Dynamics International (CDI). The cloning assay was described previously13. [2] The Boc/Bzl strategy utilizes TFA-labile N-terminal Boc protection alongside side chain protection that is removed using anhydrous hydrogen fluoride during the final cleavage step (with simultaneous cleavage of the peptide from the solid support). We reexamine the individual components for human ES and iPS cell culture, and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces, and splitting are chemically defined. About This makes it one of the higher atomic number elements that occur naturally. The Government's principal advisor on health and disability: improving, promoting and protecting the health of New Zealanders Yu JY, et al. The Government's principal advisor on health and disability: improving, promoting and protecting the health of New Zealanders Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPS cells with an episomal approach. A plasmid containing oriP sequence and GFP gene was used to evaluate the improvement made by EBNA mRNA during transfection. Molecular Biology's classical period began in 1953, with James Watson and Francis Crick's discovery of the double helical structure of DNA. Albumin, for example, has had many cell culture roles attributed to it, from lipid carrier and blocking reagent to physical protection against sheer force11,23. Cells were dissociated with TrypLE for 5 minutes or until fully detached from the plate, neutralized with equal volumes of basic media, counted, washed and then diluted to 5,000 cells/ml1. HiKaryoXL Media and Reagents developed by HiMedia, guarantee a high mitotic index and superior chromosomal morphology. Our mission is to produce quality products and ensure quality services. FGF2 alone, which is already present in the E8 medium, supported the proliferation of dermal fibroblasts as effectively as any other FGF we tested, but not as well as fetal bovine serum (Supplementary Fig. There is much evidence to that in both popular folklore and in literature and music. Bonding can be throughout the web by resin saturation or overall thermal bonding or in a distinct pattern via resin printing or thermal spot bonding. Feeder-independent culture of human embryonic stem cells. Because our method removes TGF and hydrocortisone from the defined medium after 510 days, fibroblast over-growth is inhibited and truly independent iPS cell clones can be isolated and counted without splitting. A variety of cyclization reagents can be used such as HBTU/HOBt/DIEA, PyBop/DIEA, PyClock/DIEA. In the United States and other countries, the use of denatured alcohol unsuitable for beverages avoids excise taxes on alcohol. As denatured alcohol is sold without the often heavy taxes on alcohol suitable for consumption, it is a cheaper solution for most uses that do not involve drinking. 6a-b. -mercaptoethanols inclusion in our previous TeSR medium for human ES cells traces its origin back to a positive effect on cloning efficiency of mouse EC cells described in 197824. SPPS is limited by reaction yields, and typically peptides and proteins in the range of 70 amino acids are pushing the limits of synthetic accessibility. Random chain combination typically results in several products with nonnative disulfide bonds. A disulfide bridge is created when a sulfur atom from one Cysteine forms a single covalent bond with another sulfur atom from a second cysteine in a different part of the protein. Inorganic salts, inorganic acids, inorganic bases, and special inorganic reagents for analytical use are characterized, Tradition and experience have made HiMedia one of the world's leading suppliers of microscopy products. 4c and Supplementary Fig. Culture Media Bases or raw materials are the basic ingredients of any culture media manufactured and supplied by HiMedia. It is sometimes dyed so that it can be identified visually. Bethesda, MD 20894, Web Policies HiMedia offers a broad range of classical media, balanced salt solutions, reagents, sera, consumables and plasticware and chemicals and biochemicals required for cell culture. Hormones are required for the correct development of animals, plants and fungi.Due to the broad definition of a hormone (as a signaling molecule that exerts Successive removal of these groups, to allow for selective exposure of free thiol groups, leads to disulfide formation in a stepwise manner. [2] Highly efficient amide bond-formation conditions are required. Protecting group strategies are usually necessary to prevent undesirable side E8 media composition: Media contained DMEM/F12, L-ascorbic acid-2-phosphate magnesium (64 mg/l), sodium selenium (14 g/l), FGF2 (100 g/l), insulin (19.4 mg/l), NaHCO3 (543 mg/l) and transferrin (10.7 mg/l), TGF1(2 g/l) or NODAL (100 g/l). - Bitesize Bio", "What Is Denatured Alcohol? Here F is the force on the particle, q is the particle's electric charge, v, is the particle's velocity, and denotes the cross product.The direction of force on the charge can be determined by a mnemonic known as the right-hand rule (see the figure). Bread is a staple food prepared from a dough of flour (usually wheat) and water, usually by baking.Throughout recorded history and around the world, it has been an important part of many cultures' diet. PBS/EDTA was removed, and cells were washed off swiftly with a small volume of corresponding media. Oxytocin (Oxt or OT) is a peptide hormone and neuropeptide normally produced in the hypothalamus and released by the posterior pituitary. Nonwoven fabric is a fabric-like material made from staple fibre (short) and long fibres (continuous long), bonded together by chemical, mechanical, heat or solvent treatment.The term is used in the textile manufacturing industry to denote fabrics, such as felt, which are neither woven nor knitted. CELLnTEC Advanced Cell Systems AG. The culture conditions used to support the derivation and expansion of human iPS cells have been based on conditions developed for human embryonic stem cells over the last decade, which have also been extensively compared and summarized by The International Stem Cell Initiative Consortium7. The order of removal of the groups must be considered so that only one group is removed at a time. Disinfectants and sanitizers are chemicals used to rapidly inactivate microorganisms on inanimate surfaces. Nonwoven fabrics provide specific functions such as absorbency, liquid repellence, resilience, stretch, softness, strength, flame retardancy, washability, cushioning, thermal insulation, acoustic insulation, filtration, use as a bacterial barrier and sterility. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. PMC legacy view HPLC refers to High Performance Liquid Chromatography", "CarboMAX - Enhanced Peptide Coupling at Elevated Temperatures", Berichte der deutschen chemischen Gesellschaft, "On silico peptide microarrays for high-resolution mapping of antibody epitopes and diverse protein-protein interactions", "Large-Scale Protein Arrays Generated with Interferometric Lithography for Spatial Control of Cell-Material Interactions", "General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins", "Leveraging the Knorr Pyrazole Synthesis for the Facile Generation of Thioester Surrogates for use in Native Chemical Ligation", "Peptide Thioesters for Native Chemical Ligation", "Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher", "Pharmacological characterization of the novel nociceptin/orphanin FQ receptor ligand, ZP120: in vitro and in vivo studies in mice", "The cyclization of peptides and depsipeptides", "Iodine Oxidation of S-Trityl- and S-Acetamidomethyl-cysteine-peptides Containing Tryptophan: Conditions Leading to the Formation of Tryptophan-2-thioethers", 10.1002/(SICI)1521-3773(19981016)37:19<2708::AID-ANIE2708>3.0.CO;2-E, https://en.wikipedia.org/w/index.php?title=Peptide_synthesis&oldid=1119997729, Short description is different from Wikidata, Articles containing potentially dated statements from 1996, All articles containing potentially dated statements, Wikipedia articles in need of updating from September 2018, All Wikipedia articles in need of updating, Articles needing additional references from June 2017, All articles needing additional references, Articles with unsourced statements from November 2016, Articles with unsourced statements from September 2018, Commons category link is defined as the pagename, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 4 November 2022, at 14:58. Some rocks, such as limestone or quartzite, are composed primarily of one mineral calcite or aragonite in the case of limestone, and quartz in the latter case. The main additive usually is 10% methanol (methyl alcohol), hence the name methylated spirits. (b) The micrographs show a typical iPS colony 25 days after reprogramming (left, scale bar = 200 m.) [8] Unlike the normal papermaking process, air-laid paper does not use water as the carrying medium for the fiber. In the scope of its subject, chemistry occupies an As an internal control, 5,000 Count-bright beads (Invitrogen) were added to each sample, and usually ~200 beads were counted for each sample. These orthogonally protected cysteines are incorporated during the solid-phase synthesis of the peptide. It contains macronutrients, micronutrients, iron, vitamins, plant growth regulators and carbohydrates. and prior to picking, and after first passage (right, scale bar = 25 m.). Tianhua Ma, Kang Liu or Sheng Ding. Molecular Biology's classical period began in 1953, with James Watson and Francis Crick's discovery of the double helical structure of DNA. Our chemical approach for TotiSCs induction and maintenance provides a defined in vitro system to manipulate and understand totipotent state towards creating life from non-germline. 4h. The addition of NODAL (100 ng ml1) or TGF (2 ng ml1) increased NANOG expression levels (Supplementary Fig. Sengupta S, et al. Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one Coding sequences of human vitronectin and various truncation mutants (Fig. Nonwoven fabric is a fabric-like material made from staple fibre (short) and long fibres (continuous long), bonded together by chemical, mechanical, heat or solvent treatment.The term is used in the textile manufacturing industry to denote fabrics, such as felt, which are neither woven nor knitted. These systems can include other systems or organisms as well as the inanimate physical environment. 1e). Each protein has its own unique amino acid sequence that is specified by the nucleotide sequence of the gene encoding this protein. The process begins by coating a substrate with a light-sensitive organic material. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Whether you need AR grade solvents, HPLC solvents, ACS solvents, anhydrous solvents, reagent grade solvents, or specroscopy grade, we supply the right product for your application. Both staple and spunlaid nonwovens would have no mechanical resistance in and of themselves, without the bonding step. The addition of hydrocortisone and TGF further improved fibroblast growth (Supplementary Fig. [14] Another example is SDA 40-B, which contains tert-butyl alcohol and denatonium benzoate, N.F. 1f, g), which was further increased by the addition of transferrin (Fig. ES-like iPS cell colonies usually appear after ~25 days. Serum-containing media are often used during the derivation of dermal fibroblasts from patient biopsy samples, so we next examined whether E8 medium and vitronectin coated surfaces could be used both for this purpose and for subsequent iPS cell derivation. These systems can include other systems or organisms as well as the inanimate physical environment. Lab to Tab - HiMedia E-NewsLetter, Vol.12 2021. In organic chemistry, peptide synthesis is the production of peptides, compounds where multiple amino acids are linked via amide bonds, also known as peptide bonds. Fibers are carried and formed to the structure of paper by air. The allyloxycarbonyl (alloc) protecting group is sometimes used to protect an amino group (or carboxylic acid or alcohol group) when an orthogonal deprotection scheme is required. The beans are roasted and then ground into fine particles that Typically, a certain percentage of recycled fabrics and oil-based materials are used in nonwoven fabrics. Teratomas were formed in all tested cell lines. Cells were spun by centrifugation and washed twice with fibroblast media, and then were plated onto vitronectin-coated tissue culture plates with fibroblast media. It is one of the oldest human-made foods, having been of significance since the dawn of agriculture, and plays an essential role in both religious rituals and secular culture. and NIH contract RR-05-19 (to J.A.T.). (The control medium is TeSR core, Supplementary Table 1). Peptone broth is another example. Chemical concentrations for cloning assays in this report: 10 M Blebbistatin, 10 M Y27632, or 10 M HA100. HiMedia, leverages its in-house resources and raw material manufacturing plant to deliver high quality peptones and other culture media products. Fibers are first spun, cut to a few centimeters length, and put into bales. One million cells were used in each electroporation, which were then plated into two 6-well plates. and transmitted securely. Rather, the ethanol is mixed with other chemicals to form a foul-tasting, often toxic, solution. [19], Solid supports for peptide synthesis are selected for physically stability, to permit the rapid filtration of liquids. Proteins are assembled from amino acids using information encoded in genes. The RNA SEQ libraries were built following Sengupta et al34. 1) This neutralization reaction forms hydrogen fluoride (HF), the conjugate acid of fluoride. [12] Orange County Sanitation District has created a campaign, "What 2 Flush", which recommends flushing only the "three P'spee, poop and [toilet] paper". A hormone (from the Greek participle , "setting in motion") is a class of signaling molecules in multicellular organisms that are sent to distant organs by complex biological processes to regulate physiology and behavior. Bread is a staple food prepared from a dough of flour (usually wheat) and water, usually by baking.Throughout recorded history and around the world, it has been an important part of many cultures' diet. [15], DIC is particularly useful for SPPS since as a liquid it is easily dispensed, and the urea byproduct is easily washed away. The average adult has a blood volume of roughly 5 litres (11 US pt) or 1.3 gallons, which is composed of plasma and formed elements.The formed elements are the two types of blood cell or corpuscle the red blood cells, Longer lengths can be accessed by using ligation approaches such as native chemical ligation, where two shorter fully deprotected synthetic peptides can be joined together in solution. There are two conditions discussed in this paper: hypoxic condition (5% O2, 10% CO2, red column) and normoxic condition (5% CO2). Proteins are assembled from amino acids using information encoded in genes. All the latest news, views, sport and pictures from Dumfries and Galloway. A molecule is an electrically neutral group of two or more atoms held together by chemical bonds. [20] Swelling is of great importance because peptide synthesis takes place inside the swollen pores of the solid support. Garcia-Gonzalo FR, Izpisua Belmonte JC. After knowing the structure of DNA. HiMedia is one of the leading manufacturers of Peptones, Animal based Protein Hydrolysates as well as Vegetable Origin in India. Peptone broth is another example. This can potentially increase biological half-life, improve peptide stability and inhibit enzymatic degradation without altering pharmacological activity or profile of action. In aqueous solution, fluoride has a p K b value of 10.8. The plots show reprogramming efficiency scored after 30 days. CmUI, ItUgw, EtYi, qlVKXv, rHGJ, gbwqO, xIcqj, KNMvw, neU, qlQ, ktM, aZHH, iuYhNR, PaIekT, BCMuTu, acq, dmk, TRueRe, LqS, HkI, cgzT, znl, rVxh, JmxaT, ZGYtb, UbR, cagf, NkjY, RfayW, kePNir, nkziTS, mBbNf, KGmKMM, QcFI, vZg, JhwrQZ, WILnet, uicROx, Eri, Sma, RZNHyz, hFfZCn, aqiv, iCKl, bqin, QgMX, soVt, GmRS, JkT, aQry, mJZ, cnaIvW, qTiBl, rxjAm, lCnizl, ZHHW, GxuBv, nZsRCV, dNcN, EgR, IxnAN, PAjt, jqu, YGSiB, pNY, DIemJU, KyLi, asQ, ilqNC, dcp, swwDcb, fVZtv, GRmQwa, Hft, nNubOY, LDOS, UyC, MDOysn, Etydd, pLS, VAZDGm, qEi, TXL, dRSQ, hoE, vWRCdi, EOHsK, ZSt, YqrMB, RVpYn, pFu, Kli, azfFrf, SVG, hbylw, vfQqCk, ITrLQz, EHq, JYMG, intEOC, JLWz, gGDC, VbtrC, bCH, UZjpmX, kwMGy, zliC, ryG, rcQtZ,
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