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The Control of Gene Expression (A Level only), 8.2 Regulation of Gene Expression (A Level only), 8.2.4 Producing Tissue Cultures of Explants, 8.2.6 Evaluating Data about Genetic Expression, 8.4.3 Investigating the Specificity of Restriction Enzymes, 8.4.9 Genetic Counselling & Personalised Medicine, Magnification is how many times bigger the image of a specimen observed is in compared to the actual (real-life) size of the specimen, If two separate points cannot be resolved, they will be observed as one point, As light passes through the specimen, it will be, This means that they can be much closer before the diffracted beams overlap, The concept of resolution is why the phospholipid bilayer structure of the cell membrane cannot be observed under a light microscope, The width of the phospholipid bilayer is about 10nm, The maximum resolution of a light microscope is 200nm (half the smallest wavelength of visible light, 400nm), Any points that are separated by a distance less than 200nm (such as the 10nm phospholipid bilayer) cannot be resolved by a light microscope and therefore will not be distinguishable as separate, Light microscopes are useful for looking at, The electrons are picked up by an electromagnetic lens which then shows the image, Electron microscopes are useful for looking at, Electron microscopy requires the specimen to be. 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Energy Transfers In & Between Organisms (A Level only), 1.1.10 Biochemical Tests: Sugars & Starch, 1.1.11 Finding the Concentration of Glucose, 1.3.7 The Molecular Structure of Haemoglobin, 1.3.8 The Molecular Structure of Collagen, 1.4.4 Required Practical: Measuring Enzyme Activity, 1.4.5 Maths Skill: Drawing a Graph for Enzyme Rate Experiments, 1.4.6 Maths Skill: Using a Tangent to Find Initial Rate of Reaction, 1.4.7 Limiting Factors Affecting Enzymes: Temperature, 1.4.8 Limiting Factors Affecting Enzymes: pH, 1.4.10 Limiting Factors Affecting Enzymes: Enzyme Concentration, 1.4.11 Limiting Factors Affecting Enzymes: Substrate Concentration, 1.4.12 Limiting Factors Affecting Enzymes: Inhibitors, 1.4.13 Models & Functions of Enzyme Action, 1.4.14 Practical Skill: Controlling Variables & Calculating Uncertainty, 1.5 Nucleic Acids: Structure & DNA Replication, 1.5.2 Nucleotide Structure & the Phosphodiester Bond, 1.5.6 The Origins of Research on the Genetic Code, 1.5.8 The Process of Semi-Conservative Replication, 1.5.9 Calculating the Frequency of Nucleotide Bases, 2.2.2 Microscopy & Drawing Scientific Diagrams, 2.2.6 Cell Fractionation & Ultracentrifugation, 2.2.7 Scientific Research into Cell Organelles, 2.3 Cell Division in Eukaryotic & Prokaryotic Cells, 2.3.7 Uncontrolled Cell Division & Cancer, 2.4.2 Components of Cell Surface Membranes, 2.4.8 Comparing Osmosis in Animal & Plant Cells, 2.4.13 Factors Affecting Membrane Fluidity, 2.5.5 The Role of Antigen-Presenting Cells, 2.6 Vaccines, Disease & Monoclonal Antibodies, 2.6.6 Ethical Issues with Vaccines & Monoclonal Antibodies, 3.1.5 Adaptations of Gas Exchange Surfaces, 3.2.3 Looking at the Gas Exchange under the Microscope, 3.2.11 Correlations & Causal Relationships - The Lungs, 3.4.7 Animal Adaptations For Their Environment, 3.5.8 Interpreting Data on the Cardiovascular System, 3.5.9 Correlations & Causal Relationships - The Heart, 3.5.10 Required Practical: Dissecting Mass Transport Systems, 4.2.6 Nucleic Acid & Amino Acid Sequence Comparison, 4.3 Genetic Diversity: Mutations & Meiosis, 4.3.5 Meiosis: Sources of Genetic Variation, 4.3.7 The Outcomes & Processes of Mitosis & Meiosis, 4.4.2 Maths Skill: Using Logarithms When Investigating Bacteria, 4.4.4 Directional & Stabilising Selection, 4.6.7 Quantitative Investigations of Variation, 4.6.9 Genetic Relationships Between Organisms, 5.1.1 Chloroplast Structures & their Functions, 5.1.4 Using the Products of the Light Dependent Reaction, 5.1.7 Investigating the Rate of Photosynthesis, 5.3.8 Calculating Productivity & Efficiency, 5.4.2 Practical Skill: Investigate the Effect of Minerals on Plant Growth, 5.4.3 Microorganisms Role in Recycling Minerals, 6. Technology and machinery are used with a few workers to operate them. Farmers increase the size of land for cultivation to improve yield without changing other factors. It was an era when people abandoned nomadic hunting for city settlements. Cell Structure / 2.2 The Microscope in Cell Studies / 2.2.4 Resolution & Magnification. A-Level History. About Economics (9708): A-Level History. In the UK, highland areas in the west and north allow for pastoral farming. Classification, Biodiversity & Conservation, 18.1.2 The Three Domains: Archaea, Bacteria & Eukarya, 18.2.4 Testing for Distribution & Abundance, 18.3.2 Reasons for Maintaining Biodiversity, 19.1.6 Genetic Engineering: Promoters & Marker Genes, 19.2 Genetic Technology Applied to Medicine, 19.3 Genetically Modified Organisms in Agriculture, 19.3.1 Genetically Modified Organisms in Agriculture, It is important that an excess of Benedicts solution is used so that there is more than enough copper (II) sulfate present to react with any sugar present, A positive test result is, therefore, a colour change somewhere along a colour scale from blue (no reducing sugar) to brown/brick-red (a high concentration of reducing sugar), A semi-quantitative test can be carried out by setting up standard solutions with known concentrations of reducing sugar (such as glucose), These solutions should be set up using a serial dilution of an existing stock solution, Each solution is then treated in the same way: add the same volume of Benedicts solution to each sample and heat in a water bath that has been boiled (ideally at the same temperature each time) for a set time (5 minutes or so) to allow colour changes to occur, It is important to ensure that an excess of Benedicts solution is used, Any colour change observed for each solution of a known concentration in that time can be attributed to the concentration of reducing sugar present in that solution, The same procedure is carried out on a sample with an unknown concentration of reducing sugar which is then compared to the stock solution colours to estimate the concentration of reducing sugar present, It is also possible to standardise this test but instead of waiting a fixed amount of time for a range of colours to be observed, time how long it takes for the first colour change to occur (blue to green), The higher the concentration of reducing sugar in a sample, the less time it would take for a colour change to be observed, To avoid issues with human interpretation of colour, a colourimeter could be used to measure the absorbance or transmission of light through the sugar solutions of known concentration to establish a range of values that an unknown sample can be compared against a calibration curve, Serial dilutions are created by taking a series of dilutions of a stock solution. IB Biology. Read more. Covering KS3, GCSE and A-Level, we provide the most comprehensive GCSE and A-Level revision tools to pass your exams. Lra has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning. Collections. Revision is undeniably an important factor of self-studying. ; COVID-19 vaccines are effective at protecting peopleespecially those who are boosted from getting seriously ill, being hospitalized, and even dying. Accessed 7 November, 2022. Download PDF Test Yourself. Cell Structure / 2.2 The Microscope in Cell Studies / 2.2.4 Resolution & Magnification. FREE Biology revision notes on The Microscope in Cell Studies. Helpful. A-Level Biology Tutors. Read more. Example. According to the United States Department of Agriculture (USDA), the U.S exports about 18% of all its poultry meat. 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